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2007
RoosG, Garcia-PinoA, VanBelle K, BrosensE, WahniK, VandenbusscheG, WynsL, LorisR, MessensJ.  2007.  The conserved active site proline determines the reducing power of Staphylococcus aureus thioredoxin. J. Mol. Biol.. 368(3):800-811. Abstract
2006
RoosG, BrosensE, WahniK, DesmyterA, SpinelliS, WynsL, MessensJ, LorisR.  2006.  Combining site-specific mutagenesis and seeding as a strategy to crystallize 'difficult' proteins: the case of Staphylococcus aureus thioredoxin. Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun.. 62(Pt 12):1255-1258. Abstract
RoosG, ButsL, VanBelle K, BrosensE, GeerlingsP, LorisR, WynsL, MessensJ.  2006.  Interplay between ion binding and catalysis in the thioredoxin-coupled arsenate reductase family. J. Mol. Biol.. 360(4):826-838. Abstract
VerseesW, BarlowJ, SteyaertJ.  2006.  Transition-state complex of the purine-specific nucleoside hydrolase of T. vivax: enzyme conformational changes and implications for catalysis. Journal Molecular Biology. 359(2):331-346. Abstract
2005
Dao-ThiMH, VanMelderen L, DeGenst E, AfifH, ButsL, WynsL, LorisR.  2005.  Molecular basis of gyrase poisoning by the addiction toxin CcdB. 348(5):1091-1102. Abstract
LoverixS, GeerlingsP, McNaughtonM, AugustynsK, VandemeulebrouckeA, SteyaertJ, VerseesW.  2005.  Substrate-assisted leaving group activation in enzyme-catalyzed N-glycosidic bond cleavage. J. Biol. Chem. 280(15):14799-14802. Abstract
DeGenst E, SilenceK, GhahroudiMA, DecanniereK, LorisR, KinneJ, WynsL, MuyldermansS.  2005.  Strong in vivo maturation compensates for structurally restricted H3 loops in antibody repertoires. 280(14):14114-14121. Abstract
2004
DeGenst E, HandelbergF, VanMeirhaeghe A, VynckS, LorisR, WynsL, MuyldermansS.  2004.  Chemical basis for the affinity maturation of a camel single domain antibody. 279(51):53593-53601. Abstract
MessensJ, VanMolleI, VanhaesebrouckP, LimbourgM, VanBelle K, WahniK, MartinsJC, LorisR, WynsL.  2004.  How thioredoxin can reduce a buried disulphide bond. J. Mol. Biol.. 339(3):527-537. Abstract
VerseesW, LoverixS, VandemeulebrouckeA, GeerlingsP, SteyaertJ.  2004.  Leaving group activation by aromatic stacking: an alternative to general acid catalysis. J. Mol. Biol. 338(1):1-6. Abstract
2003
DerixNM, WechselbergerRW, van der HorstMA, HellingwerfKJ, BoelensR, KapteinR, van NulandNA.  2003.  Lack of negative charge in the E46Q mutant of photoactive yellow protein prevents partial unfolding of the blue-shifted intermediate. 42(49):14501-14506. Abstract
VerseesW, SteyaertJ.  2003.  Catalysis by nucleoside hydrolases. Current opinion in structural biology. 13(6):731-738. Abstract
CanetD, LyonCE, ScheekRM, RobillardGT, DobsonCM, HorePJ, van NulandNA.  2003.  Rapid formation of non-native contacts during the folding of HPr revealed by real-time photo-CIDNP NMR and stopped-flow fluorescence experiments. 330(2):397-407. Abstract
LahN, LahJ, ZegersI, WynsL, MessensJ.  2003.  Specific potassium binding stabilizes pI258 arsenate reductase from Staphylococcus aureus. J. Biol. Chem.. 278(27):24673-24679. Abstract
AzuagaAI, CanetD, SmeenkG, BerendsR, TitgemeijerF, DuurkensR, MateoPL, ScheekRM, RobillardGT, DobsonCM, van NulandNA.  2003.  Characterization of single-tryptophan mutants of histidine-containing phosphocarrier protein: evidence for local rearrangements during folding from high concentrations of denaturant. 42(17):4883-4895. Abstract
2002
MessensJ, MartinsJC, VanBelle K, BrosensE, DesmyterA, DeGieter M, WieruszeskiJM, WillemR, WynsL, ZegersI.  2002.  All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade. Proc. Natl. Acad. Sci. USA. 99(13):8506-8511. Abstract
2001
LoverixS, SteyaertJ.  2001.  Deciphering the mechanism of RNase T1. 341:305-323.
2000
LoverixS, WinqvistA, StrombergR, SteyaertJ.  2000.  Mechanism of RNase T1: concerted triester-like phosphoryl transfer via a catalytic three-centered hydrogen bond. 7(8):651-658. Abstract
Dao-ThiMH, MessensJ, WynsL, BackmannJ.  2000.  The thermodynamic stability of the proteins of the ccd plasmid addiction system. J. Mol. Biol.. 299(5):1373-1386. Abstract
NguyenVK, HamersR, WynsL, MuyldermansS.  2000.  Camel heavy-chain antibodies: diverse germline V(H)H and specific mechanisms enlarge the antigen-binding repertoire. 19(5):921-930. Abstract
TibazarwaC, WuertzS, MergeayM, WynsL, van derLelie D.  2000.  Regulation of the cnr cobalt and nickel resistance determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34. 182(5):1399-1409. Abstract
1999
Huyghues-DespointesBM, LanghorstU, SteyaertJ, PaceCN, ScholtzJM.  1999.  Hydrogen-exchange stabilities of RNase T1 and variants with buried and solvent-exposed Ala --> Gly mutations in the helix. Biochemistry. 38(50):16481-16490. Abstract
PatteryT, HernalsteensJP, De GreveH.  1999.  Identification and molecular characterization of a novel Salmonella enteritidis pathogenicity islet encoding an ABC transporter. 33(4):791-805. Abstract
ChitiF, TaddeiN, GiannoniE, van NulandNA, RamponiG, DobsonCM.  1999.  Development of enzymatic activity during protein folding. Detection of a spectroscopically silent native-like intermediate of muscle acylphosphatase. 274(29):20151-20158. Abstract
NguyenVK, HamersR, WynsL, MuyldermansS.  1999.  Loss of splice consensus signal is responsible for the removal of the entire C(H)1 domain of the functional camel IGG2A heavy-chain antibodies. 36(8):515-524. Abstract