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Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose

Publication Type:

Journal Article

Authors:

Loris,R.; Imberty,A.; Beeckmans,S.; Van Driessche,E.; Read,J.S.; Bouckaert,J.; De Greve,H.; Buts,L.; Wyns,L.

Source:

J. Biol. Chem., Volume 278, Issue 18, p.16297 - 16303 (2003)

URL:

PM:12595543

Keywords:

Binding Sites; Crystallization; Disaccharides; chemistry; metabolism; Glucose; Molecular Conformation; Plant Lectins; Pterocarpus; Sucrose

Abstract:

The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc type specificity loop. Its metal binding loop on the other hand is of the long type, different from what is observed in other Man/Glc-specific legume lectins. Glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin A and lentil lectin. Sucrose is found to be bound in a conformation similar as seen in the binding site of lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in Pterocarpus angolensis lectin replaces a water-mediated interaction in the equivalent complex of lentil lectin. In the turanose complex, the binding site of the first molecule in the asymmetric unit contains the alphaGlc1-3betaFruf form of furanose while the second molecule contains the alphaGlc1-3betaFrup form in its binding site

Notes:

DA - 20030428
IS - 0021-9258 (Print)
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Disaccharides)
RN - 0 (Plant Lectins)
RN - 50-99-7 (Glucose)
RN - 547-25-1 (turanose)
RN - 57-50-1 (Sucrose)
SB - IM