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Location of the three disulfide bonds in an antimicrobial peptide from Amaranthus caudatus using mass spectrometry

Publication Type:

Journal Article

Authors:

el,Bouyoussfi M.; Laus,G.; Verheyden,P.; Wyns,L.; Tourwe,D.; Van,Binst G.

Source:

Volume 49, Issue 4, p.336 - 340 (1997)

URL:

PM:9176817

Keywords:

Amino Acid Sequence; Anti-Infective Agents; chemistry; Disulfides; Endopeptidases; Molecular Sequence Data; Peptide Fragments; Peptides; Seeds; Spectrometry; Mass; Fast Atom Bombardment; methods

Abstract:

The disulfide bridge pairing of Ac-AMP2, a 30-residue peptide isolated from the seeds of Amaranthus caudatus, is determined using a fast method involving enzymatic fragmentation followed by identification of the fragments with FAB mass spectrometry. The results confirm the location of the three disulfide bridges as previously established by NMR spectroscopy and molecular modelling

Notes:

DA - 19970724
IS - 1397-002X (Print)
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Anti-Infective Agents)
RN - 0 (Disulfides)
RN - 0 (Peptide Fragments)
RN - 0 (Peptides)
RN - EC 3.4.- (Endopeptidases)
SB - IM