You are hereBiblio / Upon the observation of superbeads in chromatin

Upon the observation of superbeads in chromatin

Publication Type:

Journal Article

Authors:

Muyldermans,S.; Lasters,I.; Wyns,L.; Hamers,R.

Source:

Volume 8, Issue 10, p.2165 - 2172 (1980)

URL:

PM:7433089

Keywords:

Animals; Centrifugation; Density Gradient; Chromatin; isolation & purification; metabolism; ultrastructure; Magnesium; Nucleosomes; Rats; Ribonucleoproteins; Solubility

Abstract:

There exist some indications that nucleases recognize "superbeads" in chromatin. We show that a chromatin extract of rat liver which contains so-called "superbead"-peaks can be separated in a Mg++ soluble and a Mg++ insoluble fraction. The Mg++ insoluble fraction contains the full complement of histones and the expected DNA fragments, but has lost the characteristic peaks in sucrosegradient profiles. These discrete peaks are found in the Mg2+ soluble fraction of the chromatin extract. We give evidence that these peaks are RNP particles on the basis of their protein- and nucleic acid contents

Notes:

DA - 19810126
IS - 0305-1048 (Print)
LA - eng
PT - Comparative Study
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Chromatin)
RN - 0 (Nucleosomes)
RN - 0 (Ribonucleoproteins)
RN - 7439-95-4 (Magnesium)
SB - IM