You are hereBiblio / The Shiga-toxin VT2-encoding bacteriophage phi297 integrates at a distinct position in the Escherichia coli genome
The Shiga-toxin VT2-encoding bacteriophage phi297 integrates at a distinct position in the Escherichia coli genome
Publication Type:
Journal ArticleSource:
Volume 1579, Issue 2-3, p.196 - 202 (2002)URL:
PM:12427556Keywords:
Amino Acid Sequence; Bacteriophages; Base Sequence; Binding Sites; chemistry; DNA Nucleotidyltransferases; Escherichia coli; Escherichia coli O157; Evolution; Molecular; genetics; Humans; Integrases; isolation & purification; Lysogeny; Molecular Sequence DAbstract:
The plaque-forming VT2-encoding lambdoid bacteriophage varphi297 was isolated from a Belgian clinical Escherichia coli O157:H7 isolate. PCR walking, starting from the int gene of phage varphi297, demonstrated that the varphi297 prophage integrated in the yecE gene of a lysogenic E. coli K12 strain. This integration site, in E. coli K12 and in the original clinical O157:H7 isolate, was confirmed by PCR using primers flanking this site. The excisionase protein of phage varphi297 is identical to the excisionase of VT1-encoding phage VT1-Sakai, while the integrases, which are 82% identical, show significant sequence divergence in the central and C-terminal region. This can explain the different integration sites of both prophages. The activity of the integrase was proven by its ability to mediate the integration of a suicide plasmid, carrying the attachment site of varphi297, at the appropriate position in the E. coli chromosome
Notes:
DA - 20021112 IS - 0006-3002 (Print) LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't RN - 0 (Shiga Toxin 2) RN - 0 (Viral Proteins) RN - EC 2.7.7.- (DNA Nucleotidyltransferases) RN - EC 2.7.7.- (Integrases) RN - EC 2.7.7.- (excisionase) SB - IM