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Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein

Publication Type:

Journal Article

Authors:

Steyaert,J.; Van,Melderen L.; Bernard,P.; Thi,M.H.; Loris,R.; Wyns,L.; Couturier,M.

Source:

Volume 231, Issue 2, p.513 - 515 (1993)

URL:

PM:8389886

Keywords:

analysis; antagonists & inhibitors; Bacterial Proteins; Bacterial Toxins; chemistry; Circular Dichroism; CIRCULAR-DICHROISM; Crystallization; DNA; DNA Topoisomerases; Type I; Escherichia coli; ESCHERICHIA-COLI; F Factor; isolation & purification; PROTEIN;

Abstract:

Large crystals of the Escherichia coli F plasmid CcdB killer protein were grown from solutions containing 32% ammonium sulphate. The crystals belong to space group P4(2)2(1)2 with a = b = 104.52 A and c = 88.45 A or P2(1)2(1)2(1) with a = 77.62 A, b = 93.28 A and c = 141.44 A. Both crystal forms diffract to 2.6 A resolution. Structure determination by multiple isomorphous replacement is under way

Notes:

DA - 19930712
IS - 0022-2836 (Print)
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Bacterial Proteins)
RN - 0 (Bacterial Toxins)
RN - 0 (CcdB protein, Plasmid F)
RN - EC 5.99.1.2 (DNA Topoisomerases, Type I)
SB - IM